Molds are able to reproduce sexually: Researchers grow penicillin-producing fungi with new properties

Jan. 8, 2013 ? For over 100 years, it was assumed that the penicillin-producing mould fungus Penicillium chrysogenum only reproduced asexually through spores. An international research team led by Prof. Dr. Ulrich K?ck and Julia B?hm from the Chair of General and Molecular Botany at the Ruhr-Universit?t has now shown for the first time that the fungus also has a sexual cycle, i.e. two "genders."

Through sexual reproduction of P. chrysogenum, the researchers generated fungal strains with new biotechnologically relevant properties -- such as high penicillin production without the contaminating chrysogenin. The team from Bochum, G?ttingen, Nottingham (England), Kundl (Austria) and Sandoz GmbH reports in the Proceedings of the National Academy of Sciences. The article will be published in this week's Online Early Edition.

Only penicillin producer

About 100 years ago, Alexander Fleming demonstrated the formation of penicillin in Penicillium chrysogenum. To date, there is no other known producer of the antibiotic penicillin, which has an annual global market value of about six billion Euros.

Combining genes and breeding offspring with new properties

Not only animals and plants, but also many microorganisms such as fungi and algae can reproduce sexually. The advantage: the progenies possess a combination of genes from both mating partners and thus have new properties. Sexual reproduction in fungi is, however, not the rule. Most reproduce via spores which, in the case of moulds, occur as white, green or black deposits on spoiled food. These spores only bear the genes of one parent fungus. "Five years ago we already detected the existence of so-called sex genes in Penicillium chrysogenum", says Prof. K?ck. Now, the researchers have discovered specific environmental conditions in which the fungus actually reproduces sexually. The decisive thing was to breed fungal strains in the dark under oxygen deprivation conditions in a nutrient medium supplemented with the vitamin biotin. The offspring exhibited new properties, both at the molecular level, as well as in their phenotypes.

Results could be applicable to other fungi

Using so-called microarray analysis, the biologists also investigated the activity of all the approximately 12,000 genes of the mould fungus. The result: the sex genes control the activity of biologically relevant genes, for example those for penicillin production. "We presume that the findings can also be applied to other fungi," says Ulrich K?ck, "such as Penicillium citrinum and Aspergillus terreus that produce cholesterol-lowering statins, or Penicillium brevicompactum and Tolypocladium inflatum, which produce immunosuppressives that are used in all organ transplantations." The researchers conducted the work in the Christian Doppler Laboratory "Biotechnology of Fungi" at the Ruhr-Universit?t with funding from the Christian Doppler Society (Vienna).

Share this story on Facebook, Twitter, and Google:

Other social bookmarking and sharing tools:

---

Story Source:

The above story is reprinted from materials provided by Ruhr-Universitaet-Bochum.

Note: Materials may be edited for content and length. For further information, please contact the source cited above.

---

Journal Reference:

1. J. B?hm, B. Hoff, C.M. O?Gorman, S. Wolfers, V. Klix, D. Binger, I. Zadra, H. K?rnsteiner, S. P?ggeler, P.S. Dyer, U. K?ck. Sexual reproduction and mating-type -- mediated strain development in the penicillin-producing fungus Penicillium chrysogenum. Proceedings of the National Academy of Sciences, 2013; DOI: 10.1073/pnas.1217943110

Note: If no author is given, the source is cited instead.

Disclaimer: This article is not intended to provide medical advice, diagnosis or treatment. Views expressed here do not necessarily reflect those of ScienceDaily or its staff.

Source: http://feeds.sciencedaily.com/~r/sciencedaily/top_news/~3/POnxO8Cpv9w/130108084138.htm

stacy francis tournament brackets 2012 ncaa basketball tournament walt what time is it current time a thousand words